Sexual differences in neuronal and synaptic properties across subregions of the mouse insular cortex

Background The insular cortex (IC) plays a pivotal role in processing interoceptive and emotional information, offering insights into sex differences in behavior and cognition. The IC comprises two distinct subregions: the anterior insular cortex (aIC), that processes emotional and social signals, and the posterior insular cortex (pIC), specialized in interoception and perception of pain. Pyramidal projection neurons within the IC integrate multimodal sensory inputs, influencing behavior and cognition. Despite previous research focusing on neuronal connectivity and transcriptomics, there has been a gap in understanding pyramidal neurons characteristics across subregions and between sexes. Methods Adult male and female C57Bl/6J mice were sacrificed and tissue containing the IC was collected for ex vivo slice electrophysiology recordings that examined baseline sex differences in synaptic plasticity and transmission within aIC and pIC subregions. Results Clear differences emerged between aIC and pIC neurons in both males and females: aIC neurons exhibited distinctive features such as larger size, increased hyperpolarization, and a higher rheobase compared to their pIC counterparts. Furthermore, we observed variations in neuronal excitability linked to sex, with male pIC neurons displaying a greater level of excitability than their female counterparts. We also identified region-specific differences in excitatory and inhibitory synaptic activity and the balance between excitation and inhibition in both male and female mice. Adult females demonstrated greater synaptic strength and maximum response in the aIC compared to the pIC. Lastly, synaptic long-term potentiation occurred in both subregions in males but was specific to the aIC in females. Conclusions We conclude that there are sex differences in synaptic plasticity and excitatory transmission in IC subregions, and that distinct properties of IC pyramidal neurons between sexes could contribute to differences in behavior and cognition between males and females. Supplementary Information The online version contains supplementary material available at 10.1186/s13293-024-00593-4.


Introduction
The human insula, a deep brain region with extensive connections to various cortical and subcortical regions, is known for its role in processing interoceptive and emotional information [1].The insular cortex (IC), is subdivided into two distinct regions: the anterior (aIC) and the posterior IC (pIC), each with unique functions.In humans, the aIC is associated primarily with self-awareness and social emotions like empathy and compassion [2], while the pIC handles interoceptive information processing, such as temperature and heart regulation, taste perception, pain processing, and attention to bodily sensations [3,4].Animal studies, principally rodents, also corroborate the central role played by the IC in integrating sensory, emotional, and cognitive information.The IC may contribute to sex differences in brain disorders through its involvement in various functions and its differential activation and connectivity patterns between males and females.For example, sex-related IC differences may lead to differences in how emotions are processed and how sensitive individuals are to social emotions, which are mainly associated with the aIC or on pain processing and interoceptive awareness, functions primarily associated with the pIC.These variations could result in differences in how pain is perceived, how stress is responded to, or the degree of attention given to bodily sensations when comparing individuals of different sexes.A current hypothesis is that these differences play a role in the varying prevalence and manifestation of certain disorders between the sexes.Several neuropsychiatric diseases have shown variations in prevalence between men and women.While autism spectrum disorder (ASD), attention-deficit/hyperactivity disorder (ADHD), early onset schizophrenia, conduct disorders Tourette syndrome, and substance use disorders (SUD) are more commonly diagnosed in males [5][6][7]; anxiety and traumarelated disorders, are more common in women than in men [8][9][10].Interestingly, the IC has been implicated in mood disorders, panic disorder, PTSD, obsessive-compulsive disorder, eating disorders, schizophrenia [11] and SUD [6,7].Exploring these sex-related variations in the IC's subregions is imperative to gain insights into the neural underpinnings of behaviors and cognitive processes specific to each sex.Pyramidal neurons, acting as the primary excitatory neurons within the IC, have a pivotal role in processing interoceptive, emotional, and cognitive information.Their critical function involves transmitting and integrating information within the IC, particularly from the pIC to the aIC, and onwards to output regions, thereby directly influencing both behavior and cognition.Although prior research has extensively examined the connectivity and transcriptomics of these neurons [12,13] a comprehensive investigation into the intrinsic passive, active, and synaptic properties of these neurons has been notably lacking.Yet, it is these intrinsic properties that form the very foundation of the IC's functionalities.To gain a comprehensive understanding of the IC's operations, it is imperative to systematically compare these intrinsic properties not only between different subregions but also between different sexes.This study focused on examining disparities in cellular and synaptic properties among neurons located in the aIC and pIC regions of mice, taking into consideration variations linked to sex.The results highlighted notable intrinsic dissimilarities between neurons in both the aIC and pIC across both male and female subjects.Moreover, we identified sex-related distinctions in terms of neuronal excitability and synaptic activity.These findings provide valuable insights into the neural underpinnings of sexrelated differences in behavior and cognition associated with the IC.

Animals
Animals were treated in compliance with the European Communities Council Directive (86/609/EEC) and the United States NIH Guide for the Care and Use of Laboratory Animals.The French Ethical committee authorized the project APAFIS#18476-2019022510121076 v3.Adult male and female C57BL6/J (12-17 weeks age) were purchased from Charles River and housed in standard wiretopped Plexiglas cages (42 × 27 × 14 cm), in a temperature and humidity-controlled condition (i.e., temperature 21 ± 1 °C, 60 ± 10% relative humidity and 12 h light/dark cycles).Food and water were available ad libitum.

Slice preparation
Adult male and female mice (PND 90-120, N = 16 and N = 12 respectively) were deeply anesthetized with isoflurane and sacrificed according to institutional regulations.The brain was sliced (300 μm) in the coronal plane with a vibratome (Integraslice, Campden Instruments) in a sucrose-based solution at 4 °C (87 mM NaCl, 75 mM sucrose, 25 mM glucose, 2.5 mM KCl, 4 mM MgCl 2 , 0.5 mM CaCl 2 , 23 mM NaHCO 3 , and 1.25 mM NaH 2 PO 4 ).Immediately after cutting, slices containing anterior or posterior IC were stored for 30 min at 32 °C in a low-calcium artificial CSF (ACSF) that contained the following: 130 mM NaCl, 11 mM glucose, 2.5 mM KCl, 2.4 mM MgCl 2 , 1.2 mM CaCl 2 , 23 mM NaHCO 3 , and 1.2 mM NaH 2 PO 4 , and were equilibrated with 95% O2/5% CO 2 and then at room temperature until the time of recording.During the recording, slices were placed in the recording chamber and continuously perfused at 2 ml/min with warm (32°-34 °C) low Ca 2 + solution.

Electrophysiology
Whole-cell patch clamp recordings were made from the soma of layer V pyramidal anterior or posterior IC neurons.The latter were visualized under a differential interference contrast microscope using an upright microscope with infrared illumination (Olympus, France).For current-clamp experiments and voltage clamp recording, patch pipettes were filled with an intracellular solution containing (in mM): K + gluconate (145 K + gluconate, 3 NaCl, 1 MgCl 2 , 1 EGTA, 0.3 CaCl 2 , 2 Na 2+ ATP, 0.3 Na + GTP, and 0.2 cAMP, buffered with 10 HEPES).The pH was adjusted to 7.25 and osmolarity to 290-300 mOsm.Electrode resistance was 2-4 MΩ.Access resistance compensation was not used, and acceptable access resistance was < 30 MΩ.The potential reference of the amplifier was adjusted to zero before breaking into the cell.Cells were held at − 70 mV.Current-voltage (I-V) curves were made by a series of hyperpolarizing to depolarizing current steps immediately after breaking into the cell.To determine rheobase a series of depolarizing current steps was applied.Spontaneous EPSCs (sEPSCs) were recorded at − 70 mV and isolated by using the GABA A receptor blocker gabazine 10 mM (SR 95531 hydrobromide; Tocris).
For extracellular field potential recordings, both stimulating and recording electrode were positioned in the layer V of a/pIC.Both fEPSP area and amplitude were analyzed.Stimulation was performed with a glass electrode filled with aCSF and the stimulus intensity was adjusted ~ 60% of maximal intensity after performing an input-output curve in presence of gabazine (10 mM).For LTP experiments, baseline stimulation frequency was set at 0.1 Hz and plasticity was induced by a high-frequency stimulation (HFS; stimuli were applied at 100 Hz for 1 s, repeated 3 times at 10 s interval).The glutamatergic nature of the field EPSP (fEPSP) was systematically confirmed at the end of the experiments using the ionotropic glutamate receptor antagonist CNQX (20 mM), which specifically blocked the synaptic component without altering the non-synaptic.
Data was recorded in current clamp with an Axopatch-200B amplifier, low pass filtered at 2 kHz, digitized (10 kHz, DigiData 1440A, Axon Instruments), collected and analyzed using Clampex 10.7 (Molecular Device).Membrane capacitance (Cm) was estimated by integrating the capacitive current evoked by a − 2 mV pulse, whereas the membrane resistance was estimated from the I-V curve around resting membrane potential.The latter (RMP) was measured immediately after wholecell formation during the current-clamp protocol.The input-output curve was made by measuring the number of action potentials elicited by depolarizing current steps of increasing amplitude, while to determine rheobase a series of depolarizing 10 pA current steps was applied.The frequency and amplitude of sE/ IPSCs were analysed with Axograph X using a double exponential template: f(t) = exp(−t/rise) + exp(−t/ decay) (rise = 0.5 ms and decay = 3 ms; rise = 0.2 ms and decay = 10 ms, respectively).The detection threshold for the events was set at 3 times the baseline noise SD, whereas the one for the amplitude detection was set at − 7 pA.

Data analysis and statistics
Total charge was calculated by summing the charge transfer of all individual events (sEPSCs or sIPSCs) detected over a 6 min acquisition period for each neuron.
For field recording experiments, the magnitude of plasticity was calculated at 0-10 and 20-30 min after induction as percentage of baseline responses.
Considering the parameters reported in the evaluation of intrinsic and synaptic transmission properties, PCA was computed using "The FactoMineR package".Intrinsic properties of layer 5 pyramidal neurons were analysed via PCA with membrane capacitance, rheobase, resting membrane potentials, neuronal excitabilities and voltage membrane response to a different injected current steps as quantitative variables and individual cells as individuals.Missing values were imputed by the mean of the variable.Supplementary qualitive variables were the two insular cortexes (anterior and posterior, two modalities), sex (2 modalities) and group (4 modalities).The cumulative relative contribution of PCs against the variance, the contribution and correlation were investigated.

Intrinsic properties of pyramidal neurons in the anterior and posterior IC of both sexes
In the rostro-caudal axis, the IC is divided into two regions: the anterior insula (aIC) and the posterior insula (pIC).While the distinct reciprocal connections of these regions with cortical and subcortical brain areas have been well-characterized, less is known about their intrinsic properties.Thus, electrophysiological characterizations of pyramidal neurons in both aIC and pIC were conducted in male and female subjects: layer V pyramidal neurons were whole-cell patch clamped in acute coronal slices from mouse aged between 90 to 120 postnatal days (PNDs) (Fig. 1A, B).
There were no significant differences in passive or active membrane properties when comparing males Fig. 1 Subregional intrinsic properties of anterior and posterior insular cortex pyramidal neurons in males and females.A, B Representative coronal sections of the brain from the anterior (Bregma + 1.98) and posterior (Bregma − 1.06) regions of the insular cortex (IC) showing recording sites of layer V pyramidal neurons.C, D Quantitative analysis of passive and active membrane properties revealed that anterior IC (aIC) pyramidal neurons are significantly larger (i.e., larger capacitance) and hyperpolarized compared to posterior IC (pIC) neurons, and that this difference is present in both male and female mouse.E, F Subregional differences in the current-voltage relationship between aIC and pIC pyramidal neurons were found in response to current injection steps of 50 pA, ranging from − 400 pA to + 50 pA, in adult females (E), but not in males (F).G An example of an action potential (AP) evoked by increasing current steps is illustrated, with the rheobase indicated.H The results indicated that the minimal current required to trigger an AP (rheobase) was significantly higher in aIC compared to pIC pyramidal neurons.Data are presented as box-and-whisker plots (minimum, maximum, median) for (C-D-H), and as mean ± SEM in XY plots for (E-F).Two-way ANOVA followed by Šídák's multiple comparison test was performed for (C-D-H), and Mann-Whitney U test was applied for (E-F).P-values < 0.05 are depicted in the graphs.The sample sizes for aIC male and female were 14/10 and 13/6, respectively, and for pIC male and female were 28/15 and 20/12, respectively (See figure on next page.)and females within each IC region.However, substantial differences were observed between the anterior and posterior IC.Specifically, pyramidal neurons in the aIC were notably bigger and hyperpolarized compared to those in the pIC (Fig. 1C, D, Table 1).Furthermore, aIC exhibited a higher rheobase compared to pIC neurons in both males and females, consistent with their hyperpolarized resting potential (Fig. 1H, Table 1).
When comparing the membrane voltage response to hyperpolarizing current steps, we observed that in males, there were no significant differences between aIC and pIC (Fig. 1E, Table 2).However, in females, the voltage response of aIC was significantly lower compared to pIC (Fig. 1F, Table 2).Finally, no sex differences were observed when comparing the voltage membrane responses in both cortexes (Additional file 1: Fig. S1A, B, Additional file 4: Table S1).Principal Component Analysis (PCA, Fig. 3, Table 3) with membrane capacitance, rheobase, resting membrane potentials, neuronal excitabilities, and voltage membrane response to a different injected current steps as quantitative variables, indicated that subregion if the principal contributor to the dataset variance when all groups are considered (Fig. 3B).

Excitability differences in anterior and posterior insular cortex pyramidal neurons by sex and subregion
The observed differences in passive and active membrane properties between the aIC and pIC suggest distinct levels of excitability of the principal projecting neurons in these two subregions.
To assess the intrinsic excitability of IC principal neurons, we recorded their membrane response profiles in reaction to a series of somatic current steps.Both male and female mouse exhibited significant differences in membrane profiles between the aIC and pIC, indicating a higher excitability of pyramidal neurons in the posterior IC (Fig. 2A, B, Table 4).However, when comparing sexes, pyramidal neurons showed similar excitability (Additional file 1: Fig. S1C, D, Additional file 4: Table S1).Furthermore, within the "physiological range", neurons in the aIC fired less frequently than those in the pIC, independently of sex.Notably, among males, pyramidal neurons in the posterior IC fired more frequently than in females (Fig. 2D, Table 1).Consistent with these intrinsic passive properties, PCA revealed that the two distinct subregions were the primary contributors to the variance in this dataset (see Fig. 3B).

The excitatory/ inhibitory balance of across sex and IC subregions
Alterations in excitatory (glutamatergic)/ inhibitory (GABAergic) neurotransmission in the insular cortex have been proposed to play a causal role in the development of chronic pain state and perception of painful stimuli [14].However, the E/I balance of IC subregions has not been characterized yet.First, we quantified the total charge transferred from whole-cell recorded spontaneous AMPA-mediated EPSCs (sEPSCs; Fig. 4A) and GABA-mediated IPSCs (sIPSCs; Fig. 4B) a parameter which accounts for both frequency and amplitude of spontaneous events.The total charge transfer of sEP-SCs in aIC was greater than that of the pIC in male only (Fig. 4C, Table 5).The total charge transfer of sIPSCs was similar across sexes and subregions (Fig. 4D, Table 5).Subsequently, we compared the relative distribution of sEPSCs and sIPSCs total charge transfer [15,16].Interestingly, in both sexes and subregions, sIPSCs were predominant (Fig. 4E-H) as show by the right-shift in the sIPSCs cumulative distribution, indicating that the E/I balance is shifted towards inhibition (Fig. 4E-H, Dot plots).Although the E/I balance in the IC is mainly influenced by inhibition, it is important to note that in both male and female the percentage of inhibition is larger in the pIC than in the aIC (Fig. 4E-H, Pie chart).

Subregional differences of excitatory synaptic transmission
To explore the hypothesis that synaptic connectivity is responsible for the observed subregional differences in E/I balance, we conducted recordings and compared spontaneous AMPA-mediated post-synaptic currents in layer V pyramidal neurons within both sexes and subregions (Figs.5,6).In males, it became evident that the mean amplitude of post-synaptic currents in the pIC increased, while the mean frequency was lower compared to the anterior aIC (Fig. 5C-E, Table 6.Further analysis of the frequency distribution confirmed a higher proportion of larger but less frequent excitatory events in pIC compared to aIC (Fig. 5D-F).
In contrast, for females, the mean amplitude and frequency of spontaneous excitatory post-synaptic currents (sEPSCs) remained similar in both aIC and pIC (Fig. 6C-E, Table 6).A closer examination of the frequency distribution of individual sEPSCs, however, revealed a higher proportion of smaller and more frequent synaptic currents in aIC compared to pIC in females (Fig. 6D-F, Table 6).Notably, when comparing across sexes and subregions, a sex-specific difference was observed solely in pIC.Specifically, the primary frequency of sEPSCs was higher in females than in males (Additional file 2: Fig. S2B, Additional file 5: Table S2).Finally, no significant differences were found in the kinetics of excitatory events across both subregions and sexes (Additional file 2: Fig. S2C-D, Additional file 5: Table S2).

Differential GABAergic transmission in aIC and pIC of adult males
Spontaneous GABA A -mediated inhibitory post-synaptic currents were recorded in IC principal neurons in whole-cell configuration (Figs. 7, 8).As shown in Fig. 7C, in adult male the mean amplitude of sIPSCs remained similar among the two cortexes.In contrast the frequency was lower in pIC when compared to those in aIC (Fig. 7E, Table 6).Indeed, the frequency distribution confirmed that inhibitory events were more frequent in aIC (Fig. 7F).In contrast, in female group both mean amplitude and frequency were comparable (Fig. 7C-E, Table 6).When compared across sex and subregion no further differences were found in the mean amplitude, frequency or kinetics of sIPSCs (Additional file 3: Fig. S3A-D, Additional file 5: Table S2).

LTP at the glutamatergic synapses in a sexand subregion-specific manner
The IC exhibits reciprocal connections with cortical and subcortical regions associated with sensory, cognitive, and memory functions, suggesting the involvement  Additionally, male pIC neurons showed a higher level of excitability than those of their female counterparts.E An example of firing patterns triggered by the injection of + 150 pA depolarizing currents for each group is provided.Data are presented as mean ± SEM in XY plots for (A, B), and as box-and-whisker plots (minimum, maximum, median) for (C).Mann-Whitney U test was applied for (A, B), and two-way ANOVA followed by Šídák's multiple comparison test was performed for (C).P-values < 0.05 are depicted in the graphs.The sample sizes for aIC male and female were 14/10 and 14/6, respectively, and for pIC male and female were 20/15 and 16/12, respectively of synaptic plasticity mechanisms.While Long-Term Potentiation (LTP) has been studied in the aIC, our understanding of LTP in the pIC is limited.Additionally, there is a paucity of knowledge regarding potential sex differences in synaptic processes within the IC.
Thus, excitatory post synaptic transmission in each IC were recorded in layer V pyramidal neuron in both male and female (Fig. 9A, B).We fist compared input-output (I/O) curves of fEPSP (Fig. 9C, D, Table 7).The I/O relationship did not differ between aIC and pIC in male (Fig. 9C), in contrast female showed a marked difference across subregions.Indeed, along the entire I/O curve the aIC showed a greater synaptic strength compared to the pIC (Fig. 9D).LTP could be elicited in both aIC and pIC in male (Fig. 9E, F) and in contrast, LTP could only be induced in the aIC of female (Fig. 9G, H).

Discussion
The principal findings of this study reveal distinct electrophysiological characteristics between the aIC and pIC in both males and females.These differences encompass the properties of pyramidal neurons, with aIC neurons being larger and less excitable than those in pIC.Our study highlights that the E/I balance within the IC leans towards inhibition, with pIC exhibiting a higher degree of inhibitory activity compared to aIC, regardless of sex.
Finally, there are sex-specific distinctions in synaptic plasticity.
Examination of the passive and active properties of layer V (L5) pyramidal neurons, revealed that in both sexes, principal neurons exhibit distinct characteristics along the rostro-caudal axis.Specifically, pyramidal neurons in the aIC are bigger and more hyperpolarized compared to those in the posterior pIC.Consequently, due to their different resting potentials, pyramidal neurons in aIC display a higher rheobase compared to those in pIC.
Although the voltage membrane response between aIC and pIC in males were similar, adult females exhibited a lower membrane response in aIC compared to pIC.These subregional variances in IC pyramidal neurons can be attributed to the distinct morphological and physiological properties associated with cortical neurons projecting to various cortical and subcortical targets [17].Firing patterns primarily depend on a cell's intrinsic properties [18][19][20].To investigate whether the differences in passive and active membrane properties between aIC and pIC influence the intrinsic excitability of L5 pyramidal neurons in both IC subregions, we compared their excitability.Indeed, in accordance with the more hyperpolarized resting potential and higher rheobase, pyramidal neurons in the aIC displayed lower excitability when compared to those in the pIC.Importantly, this subregional distinction in excitability was consistently observed in both males and females, suggesting that it is a shared characteristic across sexes.The results show that the IC follows a posterior-to-anterior gradient, with pIC being highly excitable and aIC being less excitable.These differences in electrophysiological properties between the aIC and pIC have functional implications for information processing within the IC.For example, the larger size and lower excitability of pyramidal neurons in the aIC suggest a potential role in integrating and processing sensory information with a higher degree of precision or selectivity.On the other hand, the smaller size and higher excitability of pyramidal neurons in the pIC may be associated with the rapid integration and relay of sensory signals to downstream targets.Given the strong association between the functional output of a brain region and the intrinsic excitability of its neurons, it's reasonable to propose that the heightened excitability observed in pIC could be linked to its function as the primary input hub for sensory signals originating from the sensory cortex and sensory thalamic nuclei [13].
The excitatory and E/I balance is a crucial factor in brain function, yet the E/I balance within the IC had not been previously characterized.Our investigation revealed a prevailing presence of inhibitory signals over excitatory ones, demonstrating a similarity in both the aIC and pIC.Notably, pIC exhibited a higher proportion of inhibitory activity in both males and females, suggesting that inhibitory processes may play a more significant role in this subregion.This inhibitory tone is believed to function as a filter for synchronizing excitatory activity, enhancing temporal precision and the dynamic range of firing to coordinate responses to sensory input [21,22].Our findings that the E/I balance within the IC (See figure on next page.)Fig. 4 Sex and subregional profiles of the Excitatory/Inhibitory (E/I) balance of the Insular Cortex.A, B Schematic illustration of synaptic charge transferred by each sEPSCs (A) and sIPSCs (B) calculated as the area inside each event as indicated by the arrows.C An analysis of the total charge of AMPA-sEPSCs measured over a 6-min period across sexes revealed similar total charges in both male and female in both the aIC and pIC.However, there was a higher total charge transfer observed in the aIC compared with pIC, but this was only observed in males.D Similarly, the total charge of GABA-sIPSCs, when measured over a 6-min period considering both area and sex, showed a comparable amount of charge transferred.This was analogous to the observations made in 'C' for AMPA-sEPSCs.E-H Cumulative frequency distribution of sEPSCs (excitation) and sIPSCs (inhibition) total charge transfer obtain from each Insular cortex within male and female animals.Insets: dot plots and pie graph showing the proportion of E versus I extrapolated at P = 0.5 from the corresponding cumulative frequency.C, D Data are presented as box-and-whisker plots (minimum, maximum, median) and analyzed via two-way ANOVA followed by Šídák's multiple comparison.P-values < 0.05 are depicted in the graphs.C The sample sizes for aIC male were 12/10 for pIC male 22/15, for aIC female 13/10 and for pIC female 12/10.D The sample sizes for aIC male were 19/11 for pIC male 13/9, for aIC female 13/10 and for pIC female 16/10 tends to lean towards inhibition is consistent with previous observations in conditions related to the IC, such as pain-related disorders [14].The prevalence of inhibitory signals over excitatory ones within the IC suggests that inhibitory processes play a significant role in regulating and coordinating the responses to sensory input.This E/I balance is crucial for maintaining the dynamic range of firing and temporal precision in sensory processing.Furthermore, the sex-specific differences in the E/I balance within the IC may indicate distinct mechanisms involved in multisensory integration between males and females.More generally, the characterization of the E/I balance within the IC has potential implications for understanding and treating sensory-related disorders.The observed predominance of inhibitory activity and the role of inhibitory signaling in filtering and modulating excitatory activity may have implications for conditions such as pain-related disorders, where aberrant sensory processing occurs.
The data also revealed a sex-specific difference in the I/O relationship.Moreover, while males exhibited comparable synaptic strength and LTP, females displayed greater synaptic strength in aIC compared to pIC.In females, the high-frequency stimulation protocol led to LTP in aIC only.These sex-specific differences in synaptic strength and LTP observed between the aIC and pIC suggest that synaptic plasticity mechanisms involved in multisensory integration may differ between males and females [23][24][25][26].Further studies aimed at elucidating these differences can provide insights into the neural mechanisms underlying sex-specific sensory processing and cognition.

Perspectives and significance
This research provides invaluable insights into the intricate role of the IC, a central hub for processing emotional and interoceptive information, which holds direct relevance in comprehending sex-based disparities in   behavior and cognition.Through the examination of distinctive properties of pyramidal neurons within both the anterior and posterior IC, our study not only underscores sex-specific variations but also unveils their potential implications on intrinsic synaptic plasticity, and excitatory transmission.These findings offer a fresh perspective on the neural underpinnings of sexrelated differences in behavior and cognition, holding the promise of extending their impact to the development of more precisely targeted interventions and treatments.This is particularly pertinent in disorders where the IC plays a pivotal role, given that these conditions often exhibit a pronounced sex bias, including prevalent instances such as anxiety, depression, and somatosensory processing disorders.
Fig. 7 Differences in inhibitory synaptic activity among male pyramidal neurons in the insular cortex, by region.A, B Representative spontaneous inhibitory postsynaptic currents (sIPSCs) recorded at − 70 mV in male aIC and pIC.C, D Upon conducting a quantitative analysis of the mean amplitude and frequency across these areas, it was found that although the amplitudes were similar, there was a lower frequency of inhibitory events in the pIC when compared to the aIC.E, F Log-normal curve fittings with confidence intervals (± CI) reveal that the amplitude and frequency distribution are skewed to the right and to the left respectively in the aIC as compared to the pIC.Data are presented as box-and-whisker plots (minimum, maximum, median) and analyzed via Mann-Whitney U test.P-values < 0.05 are depicted in the graphs.The sample size for aIC male was 22/10, and for pIC male was 14/11

Fig. 2
Fig.2Sex and subregional differences in the excitability of insular cortex pyramidal neurons.A, B As indicated by the response of action potentials to progressive depolarizing current injection, pyramidal neurons exhibit a notably higher level of excitability in the pIC compared to the aIC, and this trend is consistent across both sexes.(500 ms, ranging from 0 to 600 pA in 50 pA steps).C Representative voltage traces evoked by the injection of hyper-and depolarizing current steps (500 ms, ranging from − 150 to 250 pA in 50 pA steps) in both anterior and posterior IC pyramidal neurons of both sexes.D During physiological depolarizing current stages, neurons from the aIC displayed less frequent firing compared to those from the pIC.Additionally, male pIC neurons showed a higher level of excitability than those of their female counterparts.E An example of firing patterns triggered by the injection of + 150 pA depolarizing currents for each group is provided.Data are presented as mean ± SEM in XY plots for (A, B), and as box-and-whisker plots (minimum, maximum, median) for (C).Mann-Whitney U test was applied for (A, B), and two-way ANOVA followed by Šídák's multiple comparison test was performed for (C).P-values < 0.05 are depicted in the graphs.The sample sizes for aIC male and female were 14/10 and 14/6, respectively, and for pIC male and female were 20/15 and 16/12, respectively

Fig. 5 16 Fig. 6
Fig.5Region-specific spontaneous excitatory synaptic activity in adult male IC pyramidal neurons.A, B Representative spontaneous excitatory postsynaptic currents (sEPSCs) recorded at − 70 mV in male aIC and pIC.C, D On average, excitatory events are larger and less frequent in the pIC than the aIC.E, F Log-normal curve fittings with confidence intervals (± CI) reveal that the amplitude and frequency distribution are skewed to the right in the pIC as compared to the aIC.Data are presented as box-and-whisker plots (minimum, maximum, median) and analyzed via Mann-Whitney U test.P-values < 0.05 are depicted in the graphs.The sample size for aIC male was 14/9, and for pIC male was 27/16

Fig. 8 11 Fig. 9 Fig. 9 (
Fig. 8 Similarities in inhibitory synaptic activity among female pyramidal neurons across different subregions of the insular cortex.A, B Representative spontaneous inhibitory postsynaptic currents (sIPSCs) recorded at − 70 mV in female aIC and pIC.C, D On average, ihibitory events are comparable in amplitude and frequency in both aIC and pIC.E, F Log-normal curve fittings with confidence intervals (± CI) reveal higher proportion of smaller events in pIC compared to aIC.Data are presented as box-and-whisker plots (minimum, maximum, median) and analyzed via Mann-Whitney U test.P-values < 0.05 are depicted in the graphs.The sample size for aIC female was 15/10, and for pIC female was 19/11

Table 1
Active and passive membrane properties of aIC and pIC pyramidal neurons in both sexes

Table 2
Voltage membrane response per current steps in aIC and pIC pyramidal neurons in both sexes

Table 3
Principal component analysis (PCA) of IC pyramidal neurons intrinsic properties

Table 4
Intrinsic excitability of aIC and pIC pyramidal neurons in both sexes Principal component analysis (PCA) of intrinsic properties indicating that subregion is the main factor contributing to the variance in the dataset.This analysis was carried out using membrane capacitance, rheobase, resting membrane potentials, neuronal excitabilities, and the voltage membrane's response to varying injected current steps as quantitative variables.
A Plotting the percentage of explained variance by each PC (histogram) reveals that most of the data set's variance is explained by PC1 (65.90%), followed by PC2 (12.15%) and PC3 (5.33%).Black dots represent the cumulative percentage of explained variance.B, C PCA graphs of individuals were built with PC1 and PC2 which together explained more then 80% of the variance (see A). Small dots represent individuals colored according to their belonging to one the following qualitative supplementary variables: subregion (top), sex (below).Ellipses represent the barycenter of individuals (i.e., mean) for each category.B PCA show that the subregion is the major contributor in the overall variance.C In contrast PCA showed that in both insular cortices male and female largely overlap (See figure on next page.)

Table 5
Total charge transferred by AMPA-and GABA-mediated events in aIC and pIC of both sexes

Table 6
sE/IPSCs within the IC in both sexes